Isolation, expression and biochemical characterization of recombinant hyoscyamine-6β-hydroxylase from Brugmansia sanguinea – tuning the scopolamine production
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چکیده
منابع مشابه
Expression of Brugmansia candida Hyoscyamine 6beta-Hydroxylase gene in Saccharomyces cerevisiae and its potential use as biocatalyst
BACKGROUND Tropane alkaloids, mainly hyoscyamine and scopolamine, are widely used in medicine due to their anticholinergic activity. Scopolamine has a higher demand being the more valuable alkaloid due to its fewer side effects and higher physiological activity. Anisodamine (6beta-hydroxyhyoscyamine) is the intermediate in the conversion of hyoscyamine into scopolamine. Current studies report t...
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The isolation of phosphate solubilizing bacterial strains exhibiting high ability to solubilize soil phosphorus is a matter of great interest with high applicability. The use of phosphate solubilizing bacteria as inoculants simultaneously increases phosphate uptake by the plant and increase crop yield. Strains from the genera Pseudomonas, Bacillus and Rhizobium species are among the most powerf...
متن کاملAnalysis and sequencing of h6hmRNA, last enzyme in the tropane alkaloids pathway from anthers and hairy root cultures of Brugmansia candida (Solanaceae)
Brugmansia candida (Solanaceae) is a native tree distributed across South-American and produces the pharmacologicallyimportant group of tropane alkaloids including scopolamine. This biocompound is synthesised from hyoscyamine by action of Hyoscyamine 6-β hydroxylase (H6H, EC 1.14.11.11) at the end of the tropane alkaloid pathway. Here are reported the tissue and organ-specific expression of h6h...
متن کاملDetermination of Hyoscyamine and Scopolamine in Four Hyoscyamus Species from Iran
The purpose of the present study was to determine the tropane alkaloid content of four Hyoscyamus species from Iran, i.e. H. pusillus L., H. niger L., H. reticulatus L. and H. kurdicus Bornm. Determination of alkaloids was performed by the HPLC method. Samples were extracted with chloroform-methanol-25% ammonium hydroxide 15:5:1 (V/V/V). HPLC separation was performed on Eurospher C18 reversed p...
متن کاملDetermination of Hyoscyamine and Scopolamine in Four Hyoscyamus Species from Iran
The purpose of the present study was to determine the tropane alkaloid content of four Hyoscyamus species from Iran, i.e. H. pusillus L., H. niger L., H. reticulatus L. and H. kurdicus Bornm. Determination of alkaloids was performed by the HPLC method. Samples were extracted with chloroform-methanol-25% ammonium hydroxide 15:5:1 (V/V/V). HPLC separation was performed on Eurospher C18 reversed p...
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ژورنال
عنوان ژورنال: MedChemComm
سال: 2018
ISSN: 2040-2503,2040-2511
DOI: 10.1039/c8md00090e